Accelerating the development of pesticides and biostimulants through inclusive exploration.
Using reactive oxygen species (ROS) and chloroplast activity as indicators, we select compounds that affect the decrease/increase of plant activity. (Features) ■ By using ROS as an indicator, compounds with novel mechanisms of action can be selected with high efficiency. ■ Comprehensive exploration of pesticide types is possible (herbicidal and fungicidal activity, immune activity, environmental stress resistance). ■ Compared to conventional pesticide selection, high-throughput analysis (300 compounds/day) is possible. → Compounds that affect plant activity can be selected from small sample sizes in a single test using a 96-well plate. *ROS are produced within the plant as a result of environmental stress, defense responses, and metabolic activities. While they are important for plant life phenomena, they can also cause cellular damage due to oxidation.
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basic information
<Screening Image> Two-step selection using ROS accumulation measurement and chlorophyll autofluorescence measurement in chloroplasts ◆ Pre-treatment (1) Dispensing plant cells (green cultured cells) into a 96-well plate (2) Priming with compound addition (3) Oxidative stress treatment mimicking environmental stress ◆ Primary Screening (4) Detection of ROS accumulation using ROS luminescent reagents ◆ Secondary Screening (5) Quantification of cell damage through chloroplast autofluorescence measurement ◆ Results and Analysis Cell damage High ⇒ Herbicidal activity, bactericidal activity Low ⇒ Immune activity, stress resistance
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Applications/Examples of results
Exploration of pesticides (herbicides, fungicides, resistance inducers) and biostimulants utilizing various libraries (compounds, bioactive substances, natural extracts, food extract components).
Company information
Since our establishment, we have been supporting the commercialization of various companies. We have extensive experience and achievements in commercializing technologies developed at universities and launching new businesses utilizing subsidies. We will introduce researchers and technology seeds suitable for your company's product/technology development/research. Please feel free to contact us.
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