High-sensitivity immunoassay using nanoantibody-presenting polysaccharides [Saitama University]
By combining nanobodies with polysaccharides to create "nanobody-presenting polysaccharides," it is possible to immobilize nanobodies onto a substrate.
○Overview of the Technology We have developed a method to improve detection sensitivity using enzyme-linked immunosorbent assay (ELISA) with nanobodies. We prepared a nanobody-polysaccharide conjugate by chemically linking nanobodies to water-soluble polysaccharides, and it was confirmed that the sensitivity in the solid-phase ELISA using this conjugate is improved compared to using the nanobodies themselves. ○Comparison with Conventional Technology In ELISA using nanobodies as a substitute for conventional antibodies, there was a challenge of inactivation due to solid-phase immobilization and detachment from the substrate. This technology allows for the improvement of ELISA detection sensitivity by using water-soluble polysaccharides as a scaffold for solid-phase immobilization of nanobodies. ○Features of the Technology - Application of nanobodies in sandwich ELISA - Water-soluble polysaccharides protect nanobodies - Improved binding affinity due to nanobody aggregation - Suppression of non-specific adsorption
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This technology is a research outcome from Saitama University. We hope to collaborate with companies for practical application through joint research and development. If you have any requests for product development in collaboration with university technology, please feel free to contact us.
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(Expected Applications) - Test reagents and diagnostic drugs using nanoantibodies - Stabilization of nanoantibodies - Surface coating agents for presenting nanoantibodies on substrates (Patent Information) Japanese Patent Application No. 2017-008331 Antibody-polysaccharide conjugate and a highly sensitive immunoassay method using the same
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