After thawing, it shows a high survival rate in various cultured cells and maintains the pluripotency of stem cells (undifferentiated state).
"Cryoscaleless DMSO-Free" is a cryopreservation solution for cells that allows for approximately 90% survival rate even after thawing, while maintaining the differentiation potential of stem cells during freezing. It does not contain proteins or DMSO, and can be stored stably at 4°C for long periods. Due to the absence of toxicity from DMSO and the influence of proteins, it enables safe cryopreservation of cells with a high survival rate. 【Features】 - Does not contain serum or protein components - Approximately 90% survival rate even after thawing - Allows cryopreservation of stem cells while maintaining differentiation potential - Confirmed to be free from contamination by bacteria, fungi, and mycoplasma through sterility testing - Can be stored stably at 4°C for long periods *For more details, please refer to the related links or feel free to contact us.
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【Overview of Operation Method】 ■ Place cultured cells in a tube, centrifuge, and remove the supernatant. ■ Add this product and suspend the cells to achieve a concentration of 5×10^5 to 5×10^6 cells/ml, then aliquot 1 ml into storage tubes. ■ Freeze in a -80℃ deep freezer. ※ For long-term storage, keep in liquid nitrogen. ■ Rapidly thaw the stored cells in a 37℃ incubator while shaking, and centrifuge wash with an appropriate medium (about 10 ml). ※ For more details, please refer to the related links or feel free to contact us.
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【Usage】 ■ Freezing preservation solution for cells *For more details, please refer to the related links or feel free to contact us.
Company information
BMG is a research and development venture company that has been challenging the development of new biomaterial products adapted to trends in the medical field, based on a long-standing strong collaboration with the Kyoto University Institute for Advanced Medical Research. Focusing on the field of medical polymers (macromolecules), we are engaged in two promising growth businesses: the "BioDegmer" business and the new products business, as well as research and development aimed at providing innovative solutions in the medical field. Under the management philosophy of "contributing to the health of people around the world by working on the development of better biomaterial products and providing innovative solutions in the medical field," we support the improvement of technologies in the next generation of regenerative medicine and the healthy well-being of people in three areas.
